Deyin Guo, PhD
E-mail Address: dguo@whu.edu.cn
Office Address: 185# DongHu Road, Wuhan, Hubei, 430071
Position(s):
Professor
Affiliation(s):
Institute of Medical Virology
Research interests / Specialties:
Replication of RNA viruses (focusing on coronaviruses); innate immunity of virus infection；RNAi and genetic therapy of HIV.
Education and Training
Post-doctoral training, 1996-2001 Institute of Biotechnology, University of Helsinki, Finland
Ph.D., 1992-1995 Faculty of Biosciences, Brauschweig University of Technology, Brauschweig, Germany.
M.S.,1985-1988 Joint Program of Graduate School of Chinese Academy of Agricultural Sciences and the Institute of Microbiology of Chinese Academy of Sciences, Beijing, China.
B.S., 1981-1985 Nanjing Agricultural University, Nanjing, China
Research Description
My group is focusing on the studies on the molecular mechanisms of replication, immune escape and pathogenesis of the newly emerging coronaviruses (e.g. SARS and MERS-CoV) and the gene therapy of HIV/AIDS. For coronaviruses, we are interested mainly in RNA modifications by the virus-encoded RNA processing enzymes such as methyltransferase and exoribonuclease and antiviral screening by targeting the function and structure of the viral enzymes. For gene therapy of HIV/AIDS, we are adopting gene editing technologies to genetically modify or disrupt the HIV receptors and provirus of T cells and hematopoetic stem cells of the patients.
The nsp10-derived short peptide TP29 can suppress MHV infection.
Selected Publications:
1. Yi Wang, Ying Sun, Andong Wu, Shan Xu, Ruangang Pan, Cong Zeng, Xu Jin, Xingyi Ge, Zhengli Shi, Tero Ahola, Yu Chen*, Deyin Guo* (2015) Coronavirus nsp10/nsp16 methyltransferase can be targeted by nsp10-derived peptide in vitro and in vivo to reduce replication and pathogenesis.Journal of Virology[Epub ahead of print]
2. Lei Cui, Haiying Wang, Yanxi Ji, Jie Yang, Shan Xu, Xingyu Huang, Zidao Wang, Lei Qin, Po Tien, Xi Zhou, Deyin Guo*, Yu Chen* (2015) The Nucleocapsid protein of coronaviruses acts as a viral suppressor of RNA silencing in mammalian cells.Journal of Virology[Epub ahead of print]
3. Yu Chen, Jiali Tao, Ying Sun, Andong Wu, Ceyang Su, Guozhen Gao, Hui Cai, Su Qiu, Yingliang Wu, Tero Ahola,Deyin Guo*(2013) Structure-function analysis of SARS coronavirus RNA cap guanine-N7 methyltransferase.Journal of Virology87, 6296-305.
4. Yu Chen, Ceyang Su, Min Ke, Xu Jin, Lirong Xu, Zhou Zhang, Andong Wu, Ying Sun, Zhouning Yang, Po Tien, Tero Ahola, Yi Liang, Xinqi Liu,Deyin Guo*(2011) Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex.PLoS Pathogens7(10): e1002294. doi:10.1371/journal.ppat.1002294.
5. Yu Chen, Hui Cai, Ji’an Pan, Nian Xiang, Po Tien, Tero Ahola,Deyin Guo*(2009) Functional screen reveals SARS coronavirus nonstructural protein nsp14 as a novel cap N7 methyltransferase.Proceedings of the National Academy of Sciences of the United States of America106, 3484-9.
6. Mingzhu Zhu, Ting Fang, Shun Li, Kun Meng, Deyin Guo* (2015) Bipartite nuclear localization signal controls nuclear import and DNA binding activity of interferon regulatory factor 3.Journal of Immunology195, 289-97.
7. Weijia Cheng, Shiyou Chen, Ruiling Li, Yu Chen, Min Wang, and Deyin Guo (2015) Severe acute respiratory syndrome coronavirus protein 6 mediates ubiquitin-dependent proteosome degradation of N-Myc (and STAT) interactor. Virologica Sinica 30, 153-61.
8. Ruidong Hao, Jing He, Xing Liu, Guozhen Gao, Dan Liu, Lei Cui, Guojun Yu, Wenhui Yu, Yu Chen, Deyin Guo* (2015) Inhibition of hepatitis B virus gene expression and replication by hepatocyte nuclear factor 6.Journal of Virology89, 4345-55.
9. Ying Sun, Zidao Wang, Jiali Tao, Yi Wang, Andong Wu, Ziwen Yang, Kaimei Wang, Liqiao Shi, Yu Chen*,Deyin Guo*(2014) Yeast-based assays for the high-throughput screening of inhibitors of coronavirus RNA cap guanine-N7-methyltransferase.Antiviral Research104, 156-64.
10. Guozhen Gao, Xiaoyun Wu, Jieqiong Zhou, Mingfeng He, Johnny J. He* andDeyin Guo*(2013) Inhibition of HIV-1 transcription and replication by a newly identified cyclin T1 splice variant.Journal of Biological Chemistry288, 14297-14309.
11. Miao Jiang, Pamela Österlund, Peter Sarin, Minna Poranen, Dennis Bamford,Deyin Guo*and Ilkka Julkunen*(2011) Innate immune responses in human monocyte-derived dendritic cells are highly dependent on the size and the 5'-phosphorylation of RNA molecules.Journal of Immunology187, 1713-21.
